Eva Häffner & Frank H. Hellwig
Phylogeny of the tribe Cardueae (Compositae) with emphasis on the subtribe Carduinae:
an analysis based on ITS sequence data
Electronic supplement to:
Häffner, E. & Hellwig, F. H.: Phylogeny of the tribe Cardueae (Compositae) with emphasis
on the subtribe Carduinae: an analysis based on ITS sequence data – Willdenowia 29: 27–39. 1999.
Data matrix
coded gap character 1character 10 20 30 40 50
.........|.........|.........|.........|.........|Ancathia igniaria TCGAAGCCT--GCACAGCAGAACGACCCGTGAACACGTAATC-ACAACCGOlgaea pectinata TCGAAGCCT--GCACAGCAGAACGACCCGTGAACACGTAATC-ACAACCGSynurus deltoides TCGAAGCCT--GCATAGCAGAACGACCCGTGAACACGTAATC-ACAACCGAlfredia cernua TCGAAGCCT--GCACAGCAGAACGACCCGTGAACACGTAATC-ACAACCGOnopordum boissieri TCGAAGCCT--GCACAGCAGAACGACCCGTGAACACGTAATC-ACAACCGCarlina vulgaris TCGAAGCCT--GCAAAGCAGAACGACCCGCGAACGTGTAAAACACAACCGXeranthemum cylindraceum TCGAAGCCT--GCACAGCAAAACGACCCGCGAACATGTAACG-ACAATCGEchinops exaltatus TCGAAGCCT--GCACAGCAGAACGACCCGTGAACATGTAATG-ACAATCCBerardia subacaulis TCGAAGCCT--GCACAGCAGAACGACCCGTGAACACGTAATC-ACAACCGStemmacantha rhapontica TCGAAGCCT--GCACAGCAGAACGACCCGTGAACATGTAATC-ACAATCGSerratula coronata TCGAAGCCT--GCACAGCAGAACGACCTGCGAACACGTAATC-ACAACTGKlasea radiata TCGAAGCCT--GCACAGCAGAACGACCCGTGAACACGTAATC-ACAACCGStephanochilus omphalodes TCGAAGCCT--GCACAGCAGAACGACCCGTGAACATGTAATC-ATAACCGJurinea macrocephala TCGAAGCCT--GCATAGCAGAACGACCCGTGAACACGTAATC-ACAACAGJurinea mollis TCGAAGCCT--GCATAGCAGAACGACCCGTGAACACGTAATC-ACAACTGSaussurea riederi TCGAAGCCT--GCACAGCAGAACGACCCGCGAACACGTAATC-ACAACAGCousinia hystrix TCGAATCCT--GCATAGCAGAACGACCTGTGAACATGTAATC-ACAACCGArctium lappa TCGAAGCCT--GCACAGCAGAACGACCCGTGAACATGTAATC-ACAACCGCarduus crispus TCGAAGCCT--GCACAGCAGAACGACCCGTGGACATGTAATM-ACAGCCGCarduus nutans TCGAAGCCT--GCACAGCAGAACGACCCGTGGACATGTAATC-ACAGCCG
Carduus defloratus TCGAAGCCT--GCACAGCAGAACGACCCGTGGACATGTAATC-ACAGCCGCirsium tuberosum TCGAAGCCT--GCACAGCAGAACGACCCGTGGACACGTAATC-ACAGCCGCirsium texanum TCGAAGCCT--GCACAGCAGAACGACTCGTGGACACGTAATA-ACAGCCGTyrimnus leucographus TCGAAGCCT--GCACAGCAGAACGACCCGTGGACATGTAATC-ACAACAGCirsium ciliatum TCGAAGCCT--GCACAGCAGAACGACCCGCGAACACGTAATC-ACAGCCGCirsium eriophorum TCGAAGCCT--GCACAGCAGAACGACCCGCGAACACGTAATC-ACAGCCGNotobasis syriaca TCGAAGCCT--GCACAGCAGAACGACCCGCGAACGCGTAATC-ACA-CCCCarduus leptacanthus TTGAAGCCT--GCACAGCAGAACGACCCGTGGACACGTAATC-ACAATCGPtilostemon afer TCGAAGCCTCTGCATAGCAGAACGACCTGTGGACATGTAATC-AATTGTAGalactites duriaei TCGAAGCCTCTGCATAGCAGAATGACCTGTGGACATGTAATC-AAATGTACynara humilis TCGAAGCCT--GCACAGCAGAACGACCCGTGGACAAGTAATC-ACAGCCGLamyropsis cynaroides TCGAAGCCT--GCATCGCAGAACGACCCGTGAACACGTAATC-ACAGCCGcoded gap character 2 3 4 5 character 60 70 80 90 100 .........|.........|.........|.........|.........|Ancathia igniaria RGCGTCGTGGGGATCGAGCGTGAGCTTGAGGCCTGCGATGCTCTGTCGGCOlgaea pectinata GGTGTCGTGGGCATCGAGCGTGAGCTCGAGGCCTGCGACGCTCTGTCGGCSynurus deltoides GGCGTCGTGGGGATCGAGCGTGAGCTTGAGCCCTGCGATGCTCCGTCGGCAlfredia cernua GGCGTTGTGGGCTTCGAGCGTGAGCTCGGGGCCTATGATGCTCCGTCGGCOnopordum boissieri GGCGTCGTGGGGATCGGGTGTGAGCTTGAGCCCTGCGACGCTCCGTCGGCCarlina vulgaris -GCGGCGTGCGCTTGTGG--------------------------------Xeranthemum cylindraceum AGCGACTTGGGGATCGGGCGTTAGCCCGAAACCCTTGAAGCTATGTCGGCEchinops exaltatus -GCATCAGGGTGACCGGGTGTGAGCCTGGGAGCTGTGATGCTTTGTTGGTBerardia subacaulis GGCGTCGGGGGGATCGGGCGTGA-CGTGAGACC-GCGATGCTCCGCCGGTStemmacantha rhapontica GGTGTCGT-AGGATTGGGTGTGAGCCCTAGCCCTGCGATGCT-CGTCGGCSerratula coronata GGTGTCGT-GGGATCGGGTGTGAGCCTGAGCCTTGCGATGCT-CGCCGGCKlasea radiata CGTGTCGT-GGGATCGGGTGTGAGCCTGAGCCCTGCGATGCG-CGCCGCCStephanochilus omphalodes GGTGTCGT-GGGATCGGGTGAGAGCCTTAGTCCTACGATGCT-TGCCGACJurinea macrocephala GGTGTCGCAGGGGTCGGGTGTGAGCCCGGTCCTCGTGATGCTTTGCTGGCJurinea mollis GGTGTTGCGGGGGTCGGGTGTGAGCCTGGTCCTCGTGATGCTTTGCTGGCSaussurea riederi GGCGTCTAGGGGATCGGGTGCGAGCCCGAGCCTCGCGATGCTACGCTGGCCousinia hystrix GGCGTCGGGGGGGTCGGGTGCAAGCCTGAGCCTCGTGATGCCCTGTCGGCArctium lappa GGCATCGGGGGGATTGGGTGCGAGCCTGAGCCTTGCGATGCTTCGTCGGCCarduus crispus GGCGTCNAAGGTGTCGGGCGTGAGCCCGGTGCYTGTGATGCCTCGTCGACCarduus nutans GGCGTCAAAGGTGTCGGGCGTGAGCCCGGTGCCTGTGATGCCTTGTCGACCarduus defloratus GGCGTCAAAGGTGTCGGGCGTGAGCCCGGTGCTTGTGATGCCTCGTCGACCirsium tuberosum GGCGTCGAGGGGGTCGGGCGTGAGCCCGGTGCCYGCGATGYCTCGTCGGCCirsium texanum GGCGTCGAGGGGGTCGGGCGTTA---CG-TGCCCGCGATGCCTTGTCGACTyrimnus leucographus GGCGTCGAGGGGATCGGGCGTGAGCCTGGTCCTTGTGATGCCCTGCCGACCirsium ciliatum GGCGTCGGGGGGATCGGGTGTGAGCCCGGTGCCCGCGATGCCTCGTCGACCirsium eriophorum GGCGTCGGGGGGATCGGGTGTGAGCCCGGTGCCTGCGATGCCTCGTCGACNotobasis syriaca GGCGTTGGGGGGATCAGGCTTGAGCCTGGTGCCCGTGATGCCTCATCGACCarduus leptacanthus GGCGTCGGGGGGATCGGGTGTGAGCCCGGTGTCAGCGATGCCTTGTCGACPtilostemon afer GGTGTCAGGGGGATTGGGTGTGAGCCCGATGCCTGTGATGCCTTGCTGGTGalactites duriaei RGTGTAATGGGGATCAGGTGTGAACCTGATTCCTGTGATGCCTTGCTGGTCynara humilis GGCGTCGAGGGGGTCGGGTGTGAGCCCGATGCTCACGATGCCCCGTCGACLamyropsis cynaroides GGCTTCGGAGGGATTGGGCGTGAGCCTGTTTCCTGCAAAGCCTCGTCGGCcoded gap character 6 7 89 10 character 110 120 130 140 150 .........|.........|.........|.........|.........|Ancathia igniaria GTGCGTTTAGGGTGCCCC-TTTCG-AGGCATCATGGGCGTCAT-GTCGTCOlgaea pectinata GAGCGTTCAGGGTGCCTT-TTTTG-AGGCATCGTGGGCGTC-TTGTCGTCSynurus deltoides GTGCGTTCAGGGTGCCCC-TTTCG-AGGCATCATGGGCGTCGTCGTCGTCAlfredia cernua GTACGTTCAGG-TGCCCT-TATTG-AGGCATCATGGGCGTC-TCGTCGTCOnopordum boissieri ATGCGTTCAAGATGCCCC-TTCCGGAGGCATCATGGGCGT-ATCGTCGTCCarlina vulgaris --------------CCTC-CCTTT-GGGTGCCTCGGGTGTT-TCGTCGCCXeranthemum cylindraceum GTACGTGTGCTGTTCCAT-CTTTG-GAAAGTAGCATACGT-GC-GTTGGCEchinops exaltatus GTGCGCACACCGGGTCAC-TTTGT-GGCC-TTGTGGATGTTAT-GCCGACBerardia subacaulis GTGCGTGCACTGTGCCTC-TTTTG-AGCGATCGTGGACGTTGC-ATCGGCStemmacantha rhapontica ATGCGTCCAAGGTGCCTA-TCTTT-AGGCATCGTGGATGTT-TTGTCGGCSerratula coronata ATGCGTGCAAGGTGCCTA-TCTTT-TGGCATCGTGGATGTTGT-GTCGGCKlasea radiata ATGCGTGCAAGGTGCCTA-TCTTT-AGGCATCGAGGACGTTGT-GTCGGCStephanochilus omphalodes ATGCGTGCAAGGTGCCTA-TATGT-AGGTGCCGTGGACGTTGT-GTCGGCJurinea macrocephala ATGCATGCATGGTGTTTC-TTGCG-AGGCACTGTGTGTGTTGT-GTTGGCJurinea mollis ATGCATGCATGGTGTTTC-TTGCG-AGGCACCGTGGGTGTTGT-GTTGGCSaussurea riederi ATGCGTGCATGGTGTCCC-TTTTG-AGGCATCATGGACGTCGT-GTCGGCCousinia hystrix GTACGTGCATGGTTCCCC-TTATA-AGGCATCGTGGACGTTGC-GTTGGCArctium lappa ATACGTGCATGGTGCCCC-TTACG-AGGCATTGTGGATGTTGT-GTCGGCCarduus crispus GTGTCTCTGCGTTGCCCCGTTTTG-GGGCGTCGTGGATGTYGC-GTYGGVCarduus nutans GTGTCTCTGCGTTGCCCCGTTTTG-GGGCGTCGTGGATGTTGC-GTCGGCCarduus defloratus GTGTCTCTGCGTTGCCCCGTTTTG-GGGCGTCGTGGATGTCGC-GTCGGCCirsium tuberosum GTGCGTCTGCGATGCCCCGTTTCG-AGGCGTCGTGGATGTTGC-GTCGGCCirsium texanum GTGCGTCCGCGATGCCCCGTTTCG-CGGT-CTGTGGATGTTGC-GTCGGCTyrimnus leucographus GTGGGTCAGCAATGCCTTGTTTCA-AGGCGTCGTGGATTTTGC-GTCGGCCirsium ciliatum GTGCGTCCGCAATGCCCCGTTTCG-AGGCATCGTGGATGTTGT-GTCGGCCirsium eriophorum GTGCGTCCGCAATGCCCCGTTTCG-AGGCATCGTGGATGTTGT-GTCGGCNotobasis syriaca GTGCGTCTGCGATGCCTCGTTTCG-AGGAATCGTGGATGTTGT-GTCGGCCarduus leptacanthus GTACGTTCGTGATGCCCCGATTCG-AGGCGTCGTGGATGTTGC-GTTGGCPtilostemon afer ATGCGTGCATGGTGCCCCGATTCG-AGGCATTGTGGATGTCAT-GTCGGCGalactites duriaei ATGCGTGCATGGTGCCTCGATTTG-AGGCATTGTGGATGTCAT-GTCGGCCynara humilis GTGCGTGCATGGTGCCCCGTTTTG-AGGCATCGTGGACGTCGC-GTTGGCLamyropsis cynaroides GTGCGTTCATGTTGC-TCGTAACG-AGGCATCATGGATGTTGC-GTCGGCcoded gap character 11 12 13 character 160 170 180 190 200 .........|.........|.........|.........|.........|Ancathia igniaria ACCGAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACCAAATTTAAGOlgaea pectinata ACCGAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACCAAATTTAAGSynurus deltoides ACCGAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACCAAACTTAAGAlfredia cernua ACCGAAACAAAACCCCGGCACGGCATGTGCCAAGGAAAACAAAACTTAAGOnopordum boissieri ACCGAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACTTAAGCarlina vulgaris ACGTAAACAAA-CCCCGGCACAACACGTGCCAAGGAAAACAAAACTTAAGXeranthemum cylindraceum CCAAAAACCAA-CCCCGGCACGGCATGTGCCAAGGAAATAAAAACTTAAGEchinops exaltatus ACAAAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGBerardia subacaulis ACGAAAACAAA-CCCCGGCACG-CATGTGCCAAGGAAAACCAAACTTAAGStemmacantha rhapontica ACAAAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACTTAAGSerratula coronata ACAAAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACTTAAGKlasea radiata ACAAAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACTTAAGStephanochilus omphalodes GCATAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAACCTTAAGJurinea macrocephala ACGAAAACAAACCCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGJurinea mollis ATGATAACAAACCCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGSaussurea riederi ATGAAAACAAACCCCCGGCACGGCATGTGCCAAGGAAAACCAAACTTAAGCousinia hystrix ACAAAAACAAAACCCCGGCACGGTATGTGCCAAGGAAAACAAAACTCAAGArctium lappa ACGAAAACAAA-CCCCGGCACG-CATGTGC-AAGGAAAACCAAACTTAAGCarduus crispus ACTTWAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAGGCarduus nutans ACTTTAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAGGCarduus defloratus ACCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAGGCirsium tuberosum ACCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACCTAGGCirsium texanum ATCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAGGTyrimnus leucographus ACCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGCirsium ciliatum ACCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGCirsium eriophorum ACCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGNotobasis syriaca ACCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGCarduus leptacanthus ATCTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACAAAACATAAGPtilostemon afer ACTTAAACAAA-CCCCGGCACGGCAAGTGCCAAGGAAAATCAAACAAAAGGalactites duriaei ACCTAAACAAA-CCCCGGCACAGCAAGTGC-AAGGAAAATCAAACAAAAGCynara humilis ACGTAAACAAA-CCCCGGCACG-CATGTGC-AAGGAAAACAAAACATTAGLamyropsis cynaroides ACGTAAACAAA-CCCCGGCACGGCATGTGCCAAGGAAAACGAAATATAAGcoded gap character 14 character 210 220 230 240 250 .........|.........|.........|.........|.........|Ancathia igniaria AAGGGTRCGT-CTCGTGTAGCCCC-GTTCGCGGTGTGCGCACGTGTCGTGOlgaea pectinata AAGGGCACGT-CTCGTGTTGCCCC-GTTCGCGGTGTGCGCATGTGTCGTGSynurus deltoides AAGGGCGCGT-CTCGTGTTGTCCC-GTTCGCGGTGTGCGCACGTGTCGTGAlfredia cernua AAGGGCGCGT-CTCGTGTTGCCCC-GTTCGCGGTGTGCGCGCGTGTCGTGOnopordum boissieri AAGGGCGCGT-CTCGTGTTGCCCC-GTCCGCGGTGTGCGCACGGGCCGTGCarlina vulgaris AAGGGCGCGT-CCCGTGTTGCCCC-GTTCGCGG-GAGCGCACGGG-CGTGXeranthemum cylindraceum ATGGGCGCGT-CCGGTGTCGCCCC-GTTCTCGGTGTGCGCACAGG-CGTGEchinops exaltatus AAGGGTGCA--CCCGTGTTGCTCC-GTTCGCGGTATGCGCATGGGTCGTGBerardia subacaulis AAGGGCGCGT-CCCGTGTTGCCCC-GTTTGCGGTATGCGCATGGGCCGTGStemmacantha rhapontica AAGGGTGCGT-CTCGTGTTGTCCC-GTTTTCGGTGCGCACACAGGTCGTGSerratula coronata AAGGGTGCGT-CTTGTGTTGCCCT-GTTTTCGGTGTGCACACGGGTCGTGKlasea radiata AAGGGTGCGT-CTCGTGTTGCCCC-GTTTTCGGTGTGCACATGGGTCGTGStephanochilus omphalodes AAGGGTGCGT-CACGTGTTGTCCC-GTTTTCGGTGTGCACACGGGTTGTGJurinea macrocephala AAGGGTGCGT-CCCGTGTTGCCCC-GTTTGCGGTGTGCGCATGGGTCGTGJurinea mollis AATGGCGCGT-CCCGTGTTGCCCC-GTTCGCGGTGTGCGCGTGGGTCGTGSaussurea riederi AAGGGCGCGG-CTCGTGTTGCCCC-GTTTGCGGTGTGCACATGGGTCGTGCousinia hystrix AAGGGCGCGT-CCTGTGTCGCCTCCGTTTGCGGTGTGCGCATGGGTCGTGArctium lappa AAGGGCGCGT-CCTGTGTTGCCCC-GTTTGCGGTGTGCGCACGGGTCGTGCarduus crispus AAGGACGCGT-CTCGTGMTGCCTC-GTTCGCGGGGTGKGCAYGGGTCGTGCarduus nutans AAGGACGCGT-CTCGTGCTGCCTC-GTTCGCGGGGTGGGCACGGGTCGTGCarduus defloratus AAGGTCGCGT-CTCGTGATGCCTC-GTTCGCGGGGTGTGCATGGGTCGTGCirsium tuberosum AAGGGCGCGT-CCCGTGTTGCCCC-GTTCGCGGTGTGYGCACGGGTCGTGCirsium texanum AAGGGCGCGT-CCCGTGTTGCCCC-GTTCGCGGTGTGCGCATGGGCCGTGTyrimnus leucographus AAGGGCTTGT-CTCGTGTTGCCCC-GTTCGCGGTGTGCGCATGGGTCGTGCirsium ciliatum TAGGACGCGT-CCCGTGTCGCCCC-GTTCGCGGTGTGCGCACGGGCCGTGCirsium eriophorum TAGGACGCGT-CCCGTGTCGCCCC-GTTCGCGGTGTGCGCACGGGCCGTGNotobasis syriaca AAGGGCGCGT-CTCGTGTTGCCCC-GTTTGCGGGGTGTGCACGGGCGATGCarduus leptacanthus AAGGGCGCGT-CTCGTGTAGCCTC-GTTCGCGGTGTGCACACGGGTCGTGPtilostemon afer AAGGTCGTGT-CCCGTGTTGCCCC-GTTCGCGGTGTTTGCATGGGCTATGGalactites duriaei AAGGTCGTGT-CCCGTGTTGCCCC-GTTCGTGGTGTTTGCATGGGCTGCGCynara humilis AAGGGCGCGTGCTCGTGTCAC-TC-GTTCGCGGAGTGCAC-CGGCCCGTGLamyropsis cynaroides AAGGGTGCGT-CCTTTGTTGCCCC-GTTTGCGGTGTGCGCATGGGTCGTGcoded gap character 18 coded gap character 15 16 1719 2021 character 260 270 280 290 300 .........|...ITS1ITS2.. .....|.........|.........|Ancathia igniaria GCCTCTCATTAA--CCA?????????????????????????????????Olgaea pectinata GCCTCTCATTAAAATAAATCGCGTC-GCCTCCGACCTCGCCTCTCCCATGSynurus deltoides GCCTCTCATTAA--CCAATCGCGTC-GCCTCCGACCACGCCTCTCCCATAAlfredia cernua GCCTCTCATTAA--CCA?????????????????????????????????Onopordum boissieri GCCTCTCATTAA--CCAATCGCGTC-GCCTCCGACCACGCCTCTCCCATGCarlina vulgaris GC-TCTCTAGAA--CCAATCGCGTC-GCTCCCGACCACGCCTCCCCCACGXeranthemum cylindraceum GCCTCTTTTTAA--CCAATCGCGTC-GCTCCCGACCGC-TCTCC-CTTCGEchinops exaltatus GCCTCTTTGAAA--CCAATCGCGTC-GCCCCTGACCAT-CATCC--TATGBerardia subacaulis GCTTCTCAATAA--CCAATCGCGTC-GCCTCCGACCACGCCTCCCCATTGStemmacantha rhapontica GCCTCTCATTAA--CCAATCGCGTC-GCCCCAGACCACG-CTCCCC-ATGSerratula coronata GCCACTCAATAA--CCAATCGCGTC-GCCCTAGACCAC-CCTCCCTCATGKlasea radiata GCCTCTCAATAA--CCAATCGCGTC-GCCCCAGACCATG-CTCCCCTAAGStephanochilus omphalodes GCCTCTCATTAA--CCAATCGCGTC-GCCCCAGACCAT-TCTCCCCCATGJurinea macrocephala TCTTCTCATTAA--CCAATCGCGTC-GCGCCCCACCACGCCTCCCTTATGJurinea mollis TCTTCTCATAAAA-CCAATCGCGTC-GCCCCCGACCACGCCTCCCTCATGSaussurea riederi GTCTCTCATTAA--CCAATCGCGTC-GCCCCCAACCACGCCTCCCTCATGCousinia hystrix GCCACTCACCAA--CCAATCGCGTC-GCCCCCGACCA-GCCTCCCCTGTGArctium lappa GCCTCTCATCAAA-CCAATCGCGTC-GCCCCCGACCA--CCTCCCA-GTGCarduus crispus GCCTCTCAATAA--CCAATCGCGTG-GCCCCAGACCACGCCTCC-ATATGCarduus nutans GCCTCTYAATAA--CCAATCGCGTC-GCCCCAGACCACGCCTCC-ATATGCarduus defloratus GCCTCTCAATAA--CCAATCGCGTC-GCCCCAGACCACGCCTCC-ATACGCirsium tuberosum GCCTCTCAATAA--CCAATCGCGTC-GCCCCAGACCACGCCTCCCCAACGCirsium texanum GC-TCTCGATAA--CCAATCGCGTC-GCTCCCGACCACGCCTGCCC-ATGTyrimnus leucographus GCCTCTCAATAA--CCAATCGCGTC-GCCCCAGACCTCGCCTCCCCAACGCirsium ciliatum GCCTCTCAATAA--CCAATCGCGTCAGCCCCAGACCACGCCTCCCCAACGCirsium eriophorum GCCTCTCAATAA--CCAATCGCGTC-GCCCCAGACCATGCCTCCCCAACGNotobasis syriaca GTCCCTCAATAA--CCAATCGCGTC-GCCCTGGACCATGCCTCCCTAAAGCarduus leptacanthus GCCTCTCAATAA--CCAATCGCGTC-RCCTCAGACAACGTCTTCCCAATGPtilostemon afer GCCTCTCATTAA--CCAATCGCGTC-GCCCCAGACTATGTCTCCTCTATAGalactites duriaei GCCTCTCATTAAA-CATATCGCATC-GCCCCAGAT--TGTCTCCTCTATACynara humilis GC-TCTCAAAAA--CCAATCGCGTC-GCCCCAGACCACGACTCCTCGATGLamyropsis cynaroides ACCTCTCAATAA--CCAATCGCGTC-GCCCCAGATCAWGCACCCCCGATGcoded gap character 23 28 30 coded gap character 22 2425 2627 293132 33 34 character 310 320 330 340 350 .........|.........|.........|.........|.........|Ancathia igniaria ?????T-GCGTT-TG-GTC-GGGGG-CGGAGATTGGTCTCCCGTGTCCATOlgaea pectinata GGG-AT-GCGTT-TG-G-CCGGGGG-CGGAGATTGGTCTCCTGTGTCCATSynurus deltoides GGG-AT-GCGTT-TG-GTCCGGGG--CGGAGATTGGTCTCCTGTGTCCATAlfredia cernua ??????????????????????????????????????????????????Onopordum boissieri GGG-AC-GCGTT-TG-GTCCGGGGG-CTGAGACTGGTCTCCCGTGCCCATCarlina vulgaris GGG--TGGAGTGCCG--TC-GGGGG-CGGAGACTGGCCTCCCGTGCCCATXeranthemum cylindraceum G---AC--TGTTTTG--TC-GTGTG-CGGATATTGGTCTCCCGTTCCGATEchinops exaltatus G---AC-AAGTG--GTGTC-AGGGAGCGGATATTGGTCTCCCGTGCTCATBerardia subacaulis GG--AT-GTGTTTTG--TC-GGGGA-CGGAGACTGGTCTCCCGTGCCCATStemmacantha rhapontica GG--AT-GTGTTTTA--TCTGGGA--CGGAGACTGGTCTCCCGTGCCGACSerratula coronata GGG-AT-GTGTTTCG--TCTAGGA--CGGAGACTGGTCTCCCGTGCTGAAKlasea radiata GG--AT-GTGTTTTG--TCTGGGA--CGGAGACTGGTCTCCCGTGCCGATStephanochilus omphalodes G---AC-GTGTT-TG-GTTTGGGA--CGGAGACTGGTCTCCCGTTCCGATJurinea macrocephala GGG-AT-GGGT--TGTGTC-GGGGG-CGGAGACTGGTCTCCCGTGCCCATJurinea mollis GGA-AT-GGCT--TGTGTC-GGGA--CGGAGACTGGCCTCCCATGCCCATSaussurea riederi GGG-AC-GTGTTTTG--TTTGGGG--CGGAGAATGGTCTCCCGTGCTCATCousinia hystrix GGG-AT-GCGTCTCG--TCCGGGG--CGGAGACTGGCCTCCCGTGCCCATArctium lappa GGG-ATTGCGTGTCG--TTTGGGG--CG-AGACTGGTCTCCCGTGCCCATCarduus crispus GGGGAT-GTGTT-TG--TCTGGGG--CGGAGAATGGTCTCCCGTGCCGTCCarduus nutans GGGGAT-GTGTT-TG---CTGGGG--CGGAGAATGGTCTCCCGTGCCGTTCarduus defloratus GGGGAT-GTGTA-TG--TC-GGGG--CGGAGAATGGTCTCCCGTGCCGTCCirsium tuberosum GG--AT-GCGTT-CG--TCYGGGG--CGGAGAATGGTCTCCCGTGC-GTCCirsium texanum GGG-AT-GCGTT-CG--TCTGGGG--CGGAGAATGGTCTCCCGTGCCGTCTyrimnus leucographus GG--AT-GC-TTTCG--TCTGGGG--CGGAGAATGGTCTCCCGTGCCGTCCirsium ciliatum GG--AT-GG-TT-CG--TT-GGAG--CGGAGAATGG-CTCCCGTGCCGTTCirsium eriophorum GG--AT-GCG-CTCG--T--GGAG--CGGAGAATGGTCTCCCGTGCCGTTNotobasis syriaca TGG-AT-GCGTT-CG--TCTGGGG--CGGAGAATGGTCTCCCGTGCCGGTCarduus leptacanthus GGG-AT-CCGTT-CA--TCTGGGG--CGGAGAATGGTCTCCCGTGCCGTTPtilostemon afer GGG-AC-GTGTTTTG--TCTGGGG--CGGATACTGGTCTCCCGTGCTTCTGalactites duriaei GGG-AC-GTGTTTTG--TCTGGGG--CG-AAAATGGTCTCCCGTGCTTTTCynara humilis GGG-ATTCGGTTTTG--TCTGGGG--CG-AGACTGGTCTCCCGTGCCTTTLamyropsis cynaroides GG--AT-GCGTT-AG-GTCTGGGT--CGG-GATTGGTCTCCCGTGTCGTTcoded gap character 35 36 37 character 360 370 380 390 400 .........|.........|.........|.........|.........|Ancathia igniaria GGCGCGGTTGGCCTAAAACGGAGTCCCC-TCCGATGGACGCA-CGGCTAGOlgaea pectinata GGCGCGGTTGGCCTAAAACAGAGTCCCC-TCCGATGGACGCA-TGGCTAGSynurus deltoides GGCGCGGTTGGCCTAAAACGGAGTCCCC-TCCGATGGACGCA-TGGCTAGAlfredia cernua ??????????????????????????????????????????????????Onopordum boissieri GGC-CGGTTGGCCTAAAAAGGAGTCCCC-TCCGACGGACGCA-CGGCTAGCarlina vulgaris GGCGCGGCTGGCCCAAACAGGAGTCCCC-TTCGACGGACGCA-CGACTAGXeranthemum cylindraceum GGAGCGGTTGGCCTAAAAAAGAGTTCCC-TCCGGCAAACGCA-TGGCTAGEchinops exaltatus GGTGTGGTTGATCTAAATAGGAGTCCTC-TTCGGTGGATGCA-CGACTAGBerardia subacaulis GGCGCGGTTGGCCTAAAAAGGAGTCCCCCTTCGACGGACGCA-CGGCTAGStemmacantha rhapontica GGCGCGGTTGGCCTAAAAAGGAGTCCCC-TTTGGCGGGCGCA-CGGCTAGSerratula coronata GGCGCGGTTGGCCTAAAAAGGAGTCCCC-TTTGGCGGACGCA-CGGCTAGKlasea radiata GGTGCGGTTGGCCTAAAAAGGAGTCCCC-TTTGGCGGACGCA-CGGCTAGStephanochilus omphalodes GGTGCGGTTGGCCTAAAAAGGAGTCCCC-TTTGGCGGACGCA-CGGCTAGJurinea macrocephala GGTGTGGTTGGCCTAAAAAGGAGTCCCC-TTTGACGGACGCA-CGACTAGJurinea mollis GGTGTGGTTGGCCTAAAAAGGAGTCCCC-TTTGTCGGACGCA-CGGCTAGSaussurea riederi GGTGCGGTTGGCCTAAAAAGGAGTCCCC-TTCGACGGACGCA-CGGCTAGCousinia hystrix GGTGCGGTTGGCCTAAAAAGGAGTCCCC-TTTGACGGACGCA-CGGCTAGArctium lappa GGTGCGGTTGGCCTAAAAAGGAGTCCCC-TTTGACGGACGCA-CGGCTAGCarduus crispus GGCGCGGTTGGCCTAAAAAGGAGTCCCC-TTCGACGGACGCA-CGGCTAGCarduus nutans GGCGCGGTTGGCCTAAAAAGGAGTCCCC-TTCGACGGAGGCA-CGGCTAGCarduus defloratus GGCGCG-TTGGCCTAAAAAGGAGTCCCC-TTCGACGGATGCA-CGGCTAGCirsium tuberosum GGCGCGGTTGGCCTAAAAAGGAATCCCC-TTCGACGGACGCA-CGGCTAGCirsium texanum GGCGCGGTTGGCCTAAAAAGGAGTCCCC-TTCGACGGACGCA-CGGCTAGTyrimnus leucographus GGCGCGGTTGGCCTAAAAAAGGAGTCCC-GGCGACGGACGCA-CGGCTAGCirsium ciliatum GGCGTGGTTGGCCTAAAAAGGAGTCCCC-TTCGA-GGACGCA-CGGCTAGCirsium eriophorum GGCGTGGTTGGCCTAAAAAGGAGTCCCC-TTCGACGGACGCA-CGGCTAGNotobasis syriaca GGC----TTGGCCTAAAAAGGAGTCCCC-TTTGACGGGCGCA-CGGCTAGCarduus leptacanthus GGTGTGGTTGGCCTAAAAAGGAGTCCCC-TTCGACGGACGCA-CGGCAAGPtilostemon afer GGTGCGGTTGGCCTAAAAAGGAGTCCCT-TTTGACGGACGCA-CGGTTAGGalactites duriaei GGTGCGGTTGGCCTAAAAAGCAGTCCCT-TTTGTCGGATGCA-CGGCTAGCynara humilis GRTGCGGTTGGCCTAAAAAGGAGTCCC--TTCGGCGGATGCA-CGGCTAGLamyropsis cynaroides GGCGTGGTTGGCCTAAAAAGGAGTACC--TTCGACGGACGCAACGACTAGcoded gap character 38 39 40 41 42 character 410 420 430 440 450 .........|.........|.........|.........|.........|Ancathia igniaria TGGTGGTTGTCAAGGC-TTCGTATCGAGCCGTGCGTCGTGAGTCGTG-AGOlgaea pectinata TGGTGGTTGTCAAGGC-TTCGTATCGAGCCGTGCGTCGT-AGTCGCG-AGSynurus deltoides TGGTGGTTGTCAAGACCTTCGTATCGAGCCGTGCGTTGTGAGTCGTG-AGAlfredia cernua ??????????????????????????????????????????????????Onopordum boissieri TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGCGTCGTGAGCTGCG-AGCarlina vulgaris TGGTGGTTGTAACGGCCCTCGTATCGAGCCGTGCGTCTTGAGCCGCA-AGXeranthemum cylindraceum TGGTGGTTGTAATGGCCTTCGTATCGAGTCGTGTGTTGTGAGCCGCA-AGEchinops exaltatus TGGTGGTTGTATAATC--TCGTATCGAGCCGTGTGTTGTGAGCCGCA-AGBerardia subacaulis TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGTGTCGTGAGCCGCA-AGStemmacantha rhapontica TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGT----TGA--TGCA-AGSerratula coronata TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGC----TGA--AGCA-AGKlasea radiata TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGT----TGA--TGCA-AGStephanochilus omphalodes TGGTGGTTGTCAAAGCCTTCGTATCGAGTCGTGC----TGA--TGCT-AGJurinea macrocephala TGGTGGTTGTCGAGACCTTTGTAACGAGCCGTGC----GGA--TGCAAAGJurinea mollis TGGTGGTTGTCAAGGCCTTCGTAACGAGCCATGT-----GA--TGCAAAGSaussurea riederi TGGTGGTTTTCAAGGCCTTCGTATCGAGCTGTGC----ATA--CGCG-AGCousinia hystrix TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGC----GGA--CGCA-AGArctium lappa TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTGC-----GA--CGCA-AGCarduus crispus TGGTGGTTGAAAA-GCCTTCGTATCGAGCCGTGTGTCGTTAGCCGCATAGCarduus nutans TGGTGGTTGAAAAAGCCTTCGTATCGAGCCGTGTGTCGTTAGCCGCA-AGCarduus defloratus TGGTGGTTGATAAGGCCTTCGTATCGAGCCGTGTGTCGTTAGCCGCA-AGCirsium tuberosum TGGTGGTTGTTAAGGCCTTCGTATCGAGCCGTGTGTCGTTAGCCGCA-AGCirsium texanum TGGTGGTTGTTAAGGCCTTCGTATCGAGCCGTGCGTCGTTAGCCGCA-AGTyrimnus leucographus TGGTGGTTGATAAGTCCTTCGTCTCGAGCCATGTGTCGTTAGCCGCA-AGCirsium ciliatum TGGTGGTTGTTAAGGCCTTCGTATCGAGTCGTGCGTCGTTACGCGCA-AGCirsium eriophorum TGGTGGTTGTTAAGGCCDG-GTATCGAGTCGTGCGTCGTTAGCCGCA-AGNotobasis syriaca TGGTGGTTGTTAAGGCCTTCGTATCGAGCCGTGGGTTGTTAGTCGCA-AGCarduus leptacanthus TGG---TTGTTAAGGCCTTCGTATTGAGCTGTGCGTCGTGAGCCGCA-AGPtilostemon afer TGGTGGTTGTCAATGCCTTCGTATTGAGCCGTGTGTCTTGATCCATA-AGGalactites duriaei TGGTGGTTGTCAAGGCCTTCGTATTGAGCCGTGTGTCGTGATCTATA-AGCynara humilis TGGTGGTTGTCAAGGCCTTCGTATCGAGCCGTG-GTCGGGTGCCGCG-AGLamyropsis cynaroides TGGAGGTTTTCAAGGACTTCGTATCGAGTCGTACGCCGTGAGTCGTA-AGcoded gap character 44 coded gap character 4345 46 character 460 470 480 490 500 .........|.........|.........|.........|.........|Ancathia igniaria GTAAATGCTCTCCAAAGACCCCATTG-TGTC--CTTGCGACGATGTTTCGOlgaea pectinata GTAAACGCTCTCCAAAGACCCCAATG-TGTCGTCTTGTGACGATGCTTCGSynurus deltoides GTAAACGCTCTCCAAAGACCCCAATG-TGTCGTCTTGTGACGATGCTTCGAlfredia cernua ??????????????????????????????????????????????????Onopordum boissieri GGAAGCGCTCTCCAAAGACCCCAACG-CGTCGTCTTGCGACGATGCTTCGCarlina vulgaris GGAAGCGCTCGACAAAGACCCCAATG-CG-CGCCTCGCGACGACGCTTCGXeranthemum cylindraceum GGAATTGCTCTTTAAAGACCTTTATG-CGTCGTCTGTTGACGACGTTTCGEchinops exaltatus GGAGTTTCTCTTCAAAGACCCCTTAG-TGTCGTCTTGTGACGATGCTTCGBerardia subacaulis GGAAGCGCTCTCCAAAGACCCCAACA-CGT-GTCTTGCGACGATGCTTCGStemmacantha rhapontica GGATTTGCTCTCTAAAGACCCTAACG-TGTCGTCTTACGACGATGCTTCGSerratula coronata GGAATCGCTCTTTAAAGACCCTAACG-TGTTGTCTTACGACGATGCTTCGKlasea radiata GGAATCGCTCTCTAAAGACCCCAACG-TGT-GTCTTGCGACGATGCTTCGStephanochilus omphalodes GGAATCGCTCTCTAAAGACCCTAATG-TGTCGTTTTACGACGATGCTTCGJurinea macrocephala GGATACGCTCTATAAAGATCCCAACG-TGTCGTCTTGTGACAATGCTTCGJurinea mollis GGAT-CGCTCTAAAAAGACCCCAACG-TGCTGTCTTATGACGACACTTCGSaussurea riederi GGATTCGCTCTCCAAAGACCCCAACG-CGTCGTCTTGCGACGACGCTTCGCousinia hystrix G-AAGCGCTCTCCAAAGACCCCAA----GCCRTCTCGTGACGAC??????Arctium lappa GGAAGCGCTCTCCAATGACCCCAACG-TGTCGTCTTGCAACGACGCTTCGCarduus crispus GGAAGCGCTCTCCATAGACCCTAACG-TGTC--CTCGCGACGA-GCAACGCarduus nutans GGAAGCGCTCTACATAGACCCTAACG-TG-C--CTCGCGACGATGCAACGCarduus defloratus GGAAGCANTCTCCGTAGACCCTAATG-TGTCGTCTCGTGACGATGCTTCGCirsium tuberosum GGAAGCGCTCTCTAAAGACCCCAACG-C-TCGTCTYGCGACGAYG-TTCGCirsium texanum GGAAGCGCTCTCTAAAGACCCCAACG-TGTCGTCTCGCGACGACGCTTCGTyrimnus leucographus GGATGCGCTCTCCGAAGACCCCAACGGTGTTTTCTTGCGACGACTCTTCGCirsium ciliatum GGAAGCGCTCTCCAAAGACCCTAACG-TGTGTTCTAGCGACGATGCTTCGCirsium eriophorum GGAAGCGCTCTCCAAAGACCCTAACG-TG---TCTAGCGACGATG-TTCGNotobasis syriaca GGAAGTGCTCCCCAAAGATCCCAACG-TGTCGAATAGTGACGATGCTTCGCarduus leptacanthus GGAAGCG-TCTCCAAAGACCCCAACG-TGTCGTCTTGTGACGATGCTTCGPtilostemon afer GGAAGCGCACTCCAAATACCTCAATG-TGTCGTCTCTTGACGACACTTCGGalactites duriaei GGAAGCGCACTCCAAATACCTCAATG-TGTTGTCTCTTGATGACGTTTCGCynara humilis GGAAGCGCTCTCCATAGACCCCAACG-TGTCGTCCTGTGACGACACTTCGLamyropsis cynaroides GGAAGCGCTCTCCAAAGACCCCAACG-TGTCGTCTTGTGACGACGCTTCG 510 520 530 540 character .........|.........|.........|.........|.........gap character 0000000001111111111222222222233333333334444444 1234567890123456789012345678901234567890123456Ancathia igniaria ACC0000010001100001?00?????0001000100000100010010Olgaea pectinata ACC0000010010100000000000000001001000000100010000Synurus deltoides ACC0000010000100001000000000001000010000000010000Alfredia cernua ???0000010010000001??????????????????????????????Onopordum boissieri ACC0000010100100001000000000001000000100000010000Carlina vulgaris ACC0100?10010100111000000001000010100000000011000Xeranthemum cylindraceum ACC0000010101100101010101100000010100000000010000Echinops exaltatus ACC0100011001100001010101100001100100000100010000Berardia subacaulis ACC0001010001110001000000100000010100000000010100Stemmacantha rhapontica ACC0010110010100001001010100000010010000010110000Serratula coronata ACC0010110001100001010000000000010010000010110000Klasea radiata ACC0010110001100001000000100000010010000010110100Stephanochilus omphalodes ACC0010110001000001011001100001000010000010110000Jurinea macrocephala ACC0000010001000001000000000011100100000010100000Jurinea mollis ACC0000010001000000000000000011100110000111100000Saussurea riederi ACC0000010001000001000000000000010010000010110000Cousinia hystrix ACC000001000100000110000000000001001000001011000?Arctium lappa ACC0000010001111000110010001000010011000011110000Carduus crispus ACC0000000001100001000100010001010010000000000010Carduus nutans ACC0000000001100001000100010001011010000000011010Carduus defloratus ACC0000000001100001000100010001010110010000010000Cirsium tuberosum ACC0000000001100001000000100001010010000000010001Cirsium texanum ACC0001001001100011000010000001010010000000010000Tyrimnus leucographus ACC0000000001100001000000100100010010000000010000Cirsium ciliatum ACC0000000001100001000000100101010110000000010000Cirsium eriophorum ACC0000000001100001000000100000010110000000010111Notobasis syriaca ACC0000000001100001000000000001010010110000010000Carduus leptacanthus ACC0000000001100001000000000001010010000000010000Ptilostemon afer ACC1000000001100001000000000000010010000000010000Galactites duriaei ACC1000000001101000000000000000010011000000010000Cynara humilis ACC0000000001111011000000001000010011001000010000Lamyropsis cynaroides ACC0000000001100001000000100001000010001000010000